Characterization of five new low-molecular-mass trypsin inhibitors from white mustard (Sinapis alba L.) seed
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چکیده
منابع مشابه
Molecular Composition of Glutamine Synthetase of Sinapis alba L
Matthias Höpfner , Georg Reifferscheid, and Aloysius Wild Institut für Allgemeine Botanik der Johannes Gutenberg-Universität, Saarstraße 21, D-6500 Mainz, Bundesrepublik Deutschland Z. Naturforsch. 43c, 194-198 (1988); received November 26, 1987 Active Centers, Enzyme Purification, Enzyme Structure, Glufosinate, Glutamine Synthetase, Phosphinothricin Chloroplastic glutamine synthetase of Sinapi...
متن کاملCharacterization of Glutamine Synthetase of Roots, Etiolated Cotyledons and Green Leaves from Sinapis alba (L.)
Glutamine synthetase of roots, etiolated cotyledons and green leaves from mustard plants cannot all clearly be separated by DEAE-Sephacel chromatography. However, the enzyme of the roots, etiolated cotyledons and green leaves, respectively, differed in the kinetic properties deter mined in the crude extract. The root enzyme showed a pH-optimum of about 6.9, a Km value of 3 m M for glutamate an...
متن کاملCloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.).
Restriction fragments of chloroplast (cp)DNA from mustard leaves were cloned in E. coli using pBR 322 as the vehicle. Cloned fragments containing the structural sequences for two polypeptides of 56,000 and 35,000 daltons were selected by a mRNA hybridization-translation procedure. The cloned mustard genes are structurally related to chloroplast genes from maize. They hybridize to the maize mRNA...
متن کاملMyrosinase from Sinapis alba L.: A New Method of Purification for Glucosinolate Analyses
Myrosinase from white mustard seeds (Sinapis alba) has been purified starting from aqueous crude extract in a single step by affinity chromatography on Con A-Sepharose. The specific activity, recovery, and binding capacity in four separate trials using glucose, mannose, methyl a-D-glucoside, and methyl a-D-mannoside for elution were also determined. The enzyme isolated by our approach showed a ...
متن کاملNucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).
From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2000
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2000.01741.x